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The protein Src is phosphorylated at Y397, Y419, and Y530 by FAK. When exon 33 is deleted in FAK in cancer cells (Lane 3 below),

You are working with a protein that is polyubiquitinated (a variable number of ubiquitins), which you visualize with an anti-ubiquitin antibody. You treat the protein with an enzyme that cleaves ubiquitin from proteins and degrades it (+ lane). What would be the expected results?

A blue native PAGE is advantageous over a standard native gel because it

You’re working with an MBP-p53 fusion protein, which is linked by covalent peptide bonds. You would expect to see ______ band(s) on SDS-PAGE, and ______ band(s) on a native gel.

You attempt to express a large protein using yeast cells, but the protein did not properly fold with this system. Which of the following is the appropriate next step for achieving successful protein expression?

In the phage vector induction system,

The tracking dye is used in all of the following  ways EXCEPT

Why is a solubility test important before ion exchange chromatography?

Two proteins elute in fractions 30 and 40 of a size exclusion column on their own. You mix them in equal molar ratios and run them over the same column, and you only see peaks in fractions 30 and 40. What would you conclude?

In chromatin immunoprecipitation,