Zebrafish (Danio rerio), tropical freshwater fish, have been…

Questions

Zebrаfish (Dаniо reriо), trоpicаl freshwater fish, have been used as model organisms to study a number of biological phenomena. Two contrasting varieties of zebrafish are the wildtype and the golden mutant. These varieties contrast in pattern and pigmentation. Wildtype zebrafish have dark stripes and a grey background produced by melanophores that produce the pigment melanin. Golden zebrafish have a mutation to the gene (SLC24A5) which produces melanophores. These fish are hypopigmented since less melanin is produced as a result of the mutation, hence their golden coloration.  A previous study determined that crossing wildtype and golden zebrafish produces an F1 generation that are all similar to the wildtype, establishing the wildtype as the dominant phenotype. A. Use a Punnett square to predict the ratio of genotypes and phenotypes of the F2 generation produced from a cross between two of the F1 individuals mentioned above. Give appropriately labeled genotype and phenotype ratios as your answer. (2pts) B. Write a null and alternative hypothesis (in the correct format) for how the expected phenotype ratio will compare the observed ratio. (2pts) C. Use a χ2 analysis to determine if the number of observed fish of the 1000 fish sample given below is significantly different from the expected value. Write a statement summarizing your conclusion. (4pts)   Remember the equation for  

Zebrаfish (Dаniо reriо), trоpicаl freshwater fish, have been used as model organisms to study a number of biological phenomena. Two contrasting varieties of zebrafish are the wildtype and the golden mutant. These varieties contrast in pattern and pigmentation. Wildtype zebrafish have dark stripes and a grey background produced by melanophores that produce the pigment melanin. Golden zebrafish have a mutation to the gene (SLC24A5) which produces melanophores. These fish are hypopigmented since less melanin is produced as a result of the mutation, hence their golden coloration.  A previous study determined that crossing wildtype and golden zebrafish produces an F1 generation that are all similar to the wildtype, establishing the wildtype as the dominant phenotype. A. Use a Punnett square to predict the ratio of genotypes and phenotypes of the F2 generation produced from a cross between two of the F1 individuals mentioned above. Give appropriately labeled genotype and phenotype ratios as your answer. (2pts) B. Write a null and alternative hypothesis (in the correct format) for how the expected phenotype ratio will compare the observed ratio. (2pts) C. Use a χ2 analysis to determine if the number of observed fish of the 1000 fish sample given below is significantly different from the expected value. Write a statement summarizing your conclusion. (4pts)   Remember the equation for  

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