Blog

You want to know whether the virulence genes present in Bordetella pertussis are evolutionarily related to genes in the less pathogenic species B. bronchiseptica or if the virulence genes were acquired via horizontal gene transfer. What characteristic(s) would you compare to answer this question?

​ Medium A Medium B Medium C Medium components Glucose 10 g (NH4)2SO4 1 g NaCl 2.5 g KH2PO4 3 g Na2HPO4 6.8 g MgSO4 0.5 g Glucose 10 g (NH4)2SO4 1 g NaCl 2.5 g KH2PO4 3 g Na2HPO4 6.8 g MgSO4 0.5 g FeCl3 0.01 g Glucose 10 g (NH4)2SO4 1 g NaCl 2.5 g KH2PO4 3 g Na2HPO4 6.8 g MgSO4 0.5 g KNO3 0.5 g ​                   What term best describes all three media shown in the table above?

Using the following formulas: N = N02n  and g = t/n Find the generation time of a bacterial culture after 3 hours if the initial cell number is 3 × 106 and the final cell number is 2 × 108 (round to one significant figure).

In the lac operon, which of the following conditions would allow the structural genes to be transcribed?

Gene function is annotated based on homology between the ORFs of a genome and proteins whose function has been proven experimentally. What -omic approach could help us determine the function and structure of proteins encoded by uncharacterized ORFs?

When more than one operon is under the control of a single regulatory protein, the operons are collectively called a(n)

In E. coli, the adenine in the sequence GATC is methylated by the Dam enzyme. In the same cells, a restriction endonuclease recognizes and cleaves dsDNA with the sequence GATC on either strand. Why does E. coli have these two enzymes?

Considering the catabolite repression mechanism, which observation would make you suspect it IS occurring? In other words, which of the following would indicate repression is happening.

You have isolated two different strains of Neisseria gonorrhoeae from a patient (strain A and strain B). Strain A has pili, while strain B does not. Which one of the two strains is more likely to cause a serious infection?

Optical density and viable cell concentration are LEAST proportional to each other during the