You analyze a DNA sample which gives an A260 of 1.56 and an A280 of 0.98. What is the concentration of this DNA sample, and is it sufficiently pure?
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How do you prepare 600 ml of 50 mM Tris (Mw = 121.14)?
How do you prepare 600 ml of 50 mM Tris (Mw = 121.14)?
Which type of rotor allows you to spin samples into the best…
Which type of rotor allows you to spin samples into the best pellet possible?
You want to load DNA onto a gel which has 40 µl wells. How m…
You want to load DNA onto a gel which has 40 µl wells. How much of a 5X stock of sample loading buffer should be mixed with the DNA to fill up the well?
You add 1000 microliters of Folin reagent to a lysate sample…
You add 1000 microliters of Folin reagent to a lysate sample and read its absorbance in the spectrophotometer, which gives a reading of 0.3 compared to a blank. This is a(n) ________ measurement.
A 400 amino acid protein would weigh approximately ______ kD…
A 400 amino acid protein would weigh approximately ______ kDa.
An experiment calls for 375 ml of 55 mM Tris (Mw = 121.14 g/…
An experiment calls for 375 ml of 55 mM Tris (Mw = 121.14 g/mol), and you have a 5 M stock of Tris. How many milliliters of Tris are needed to make this solution?
Collecting data until nonsignificant results become signific…
Collecting data until nonsignificant results become significant is called
You wish to study the effects of substrate concentration on…
You wish to study the effects of substrate concentration on enzyme rate. For the experiment, you alter the substrate concentration and measure the change in enzyme rate, while making sure the enzyme concentration is constant in each sample. Which is the control?
Which type of inhibitor typically binds to the enzyme’s acti…
Which type of inhibitor typically binds to the enzyme’s active site?