You have a stock of DNA ligase at 50,000 units/µl. You need 5,000 units of ligase in a 25 µl reaction, how many microliters of ligase do you need?
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A large gathering of experts in a scientific field (such as…
A large gathering of experts in a scientific field (such as the Tuberculosis Consortium, or an Emerging Infectious Diseases Meeting) is a(n)
Which micropipette should be used for the most accurate tran…
Which micropipette should be used for the most accurate transfer of 50 microliters?
A 150 kDa protein would consist of approximately ______ amin…
A 150 kDa protein would consist of approximately ______ amino acids.
You analyze a DNA sample which gives an A260 of 1.56 and an…
You analyze a DNA sample which gives an A260 of 1.56 and an A280 of 0.98. What is the concentration of this DNA sample, and is it sufficiently pure?
How do you prepare 600 ml of 50 mM Tris (Mw = 121.14)?
How do you prepare 600 ml of 50 mM Tris (Mw = 121.14)?
Which type of rotor allows you to spin samples into the best…
Which type of rotor allows you to spin samples into the best pellet possible?
You want to load DNA onto a gel which has 40 µl wells. How m…
You want to load DNA onto a gel which has 40 µl wells. How much of a 5X stock of sample loading buffer should be mixed with the DNA to fill up the well?
You add 1000 microliters of Folin reagent to a lysate sample…
You add 1000 microliters of Folin reagent to a lysate sample and read its absorbance in the spectrophotometer, which gives a reading of 0.3 compared to a blank. This is a(n) ________ measurement.
A 400 amino acid protein would weigh approximately ______ kD…
A 400 amino acid protein would weigh approximately ______ kDa.