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In which expression system does T7 RNA polymerase enzyme transcribe the gene of interest on the plasmid into mRNA?

Which type of ELISA is most specific?

TARP protein (with domains A through D) was tested to determine its ability to polymerize actin, which will then be found in the pellet after centrifugation. Which of the following is TRUE regarding TARP?

Hydrophobic interaction chromatography works because

The advantage of the phage expression system over the Ptac expression system is that

What pH must you use to purify a protein with a pI of 4.2 via anion exchange chromatography?

A hydrophobic integral membrane protein is expressed in E. coli. After centrifugation, SDS-PAGE locates the protein in the pellet. Which chemical or technique would be best to solubilize this protein?

When the phage bacterial expression system is off,

Why can’t SDS-PAGE be used as a purification technique for large amounts of protein?

You can search for proteins that bind DNA using all of the following EXCEPT