Author: Anonymous

You are trying to separate 2 proteins by gel exclusion chromatography . Listed below is information about each protein.   Molecular Weight pI Protein 1 55 kDa 4.2 Protein 2 22 kDa 8.7 Which protein will elute first from the column? Give a reason for your choice Which other method could you use to separate this proteins? Explain

When a peptide bond is formed, a(n ) [1] group reacts with a carboxylate group.   In the peptide Trp─Ser─Val, valine is at the [2].   In the tripeptide Trp─Val─Phe, the N-terminal residue is [3].

______________ has emerged as a technique for protein sequencing.

What has the greatest influence on protein stability?

The classic experiment demonstrating that reduced and denatured RNase A could refold into the native form demonstrates that _______.

You have purified a new peptide hormone.  To determine its amino acid sequence you have digested the polypeptide with trypsin and in a separate reaction you have cleaved the polypeptide with cyanogen bromide.    Cleavage with trypsin yielded 5 peptides that were sequenced by Edman degradation as shown in the following.    Ser─Leu Asp─Val─Arg Val─Met─Glu─Lys Ser─Gln─Met─His─Lys Ile─Phe─Met─Leu─Cys─Arg   Cleavage with cyanogen bromide yielded 4 peptides that were sequenced by Edman degradation:   His─Lys─Ser─Leu Asp─Val─Arg─Val─Met Glu─Lys─Ile─Phe─Met Leu─Cys─Arg─Ser─Gln─Met   Determine the sequence of the intact protein.

Match the signal sequence or post-translation modification with the target

Which of the following does not require ATP hydrolysis to aid in protein folding?

Protein denaturation is caused by the following except

Which of the following developed during the evolution of eukaryotic cells from prokaryotic cells?