Author: Anonymous

Put the following events of transcription in chronological order. Sigma binds to the promoter region. The double helix of DNA is unwound, breaking hydrogen bonds between complementary strands. Sigma binds to RNA polymerase. Sigma is released. Transcription begins.

You have isolated a lac metabolism mutant that contains a mutation in the lacI gene.  This mutation alters its inducer binding site such that it cannot recognize lactose.  Which of the following best describes the phenotype you would expect to see in this mutant?

Label the following components on the replication fork:

For each of the following descriptions, please select all of the factors that would apply: Factor that bind to the -35 and -10 promoter sequences to recruit RNA polymerase in prokaryotes

Termination of transcription in prokaryotes involves formation of a hairpin structure in the newly-synthesized mRNA followed by a string of Us.

Low cAMP levels indicate high glucose levels in the cell.

Eukaryotes can undergo splicing and translation simultaneously.

Please match the following descriptions with the appropriate term.

Gene-specific transcription factors require the mediator complex to interact with RNA polymerase II in the transcription initiation complex in eukaryotes.

Scientists learn a lot from mutated versions of proteins.  The phenotype of a mutant can inform them about the normal function of the protein in various processes.  Predict what the consequence of the follow mutations would be: Loss of function mutation in MutS