Author: Anonymous

When expressing proteins such as insulin, yeast may be more advantageous than E. coli because

Protein X is reported to decrease p53 levels. After treating cells with Protein X (+ lane) and adding anti-p53 and control antibodies, you see the following results. Which result indicates that Protein X decreases p53 levels?

Which of the following is TRUE regarding purifying proteins using the ammonium sulfate precipitation?

Which of the following techniques has an identical setup to a western blot?

Which technique allows one to purify proteins directly from organs (such as from gizzards)?

A protein has a pI of 7, and is in a buffer at pH 5. What type of column should it bind to?

In which expression system does T7 RNA polymerase enzyme transcribe the gene of interest on the plasmid into mRNA?

Which type of ELISA is most specific?

TARP protein (with domains A through D) was tested to determine its ability to polymerize actin, which will then be found in the pellet after centrifugation. Which of the following is TRUE regarding TARP?

Hydrophobic interaction chromatography works because