Author: Anonymous

Using the standard curve below, what is the concentration of a sample that gives an absorbance of 0.3 when 6 microliters of sample are assayed?

Buffers lose their capacity to resist changes in pH when

How do you prepare 1 L of 80 mM Tris pH 8 (Mw = 121.14), 400 mM NaCl (Mw = 58.44) from a 1 M stock of Tris pH 8? 

A protein has a concentration of 30 μM, and 3 tryptophans (ε = 5500) and 2 tyrosines (ε = 1490). What is its absorbance?

Which of the following quantification assays is most variable with regards to the protein’s sequence?

The absorbance of a solution is directly proportional to

Escherichia coli is a defined organism with minimal risk of disease, and treatment involves taking antibiotics. A lab that studies this organism would be assigned a rating of

Expert molecular biologists from universities all over the country meet to discuss and present the latest molecular biology research. This would be considered a(n)

You analyze a DNA sample which gives an A260 of 1.87 and an A280 of 1.15. What is the concentration of this DNA sample, and is it sufficiently pure?

Phosphate buffers are advantageous because