Author: Anonymous

You notice that your micropipette is inconsistently aspirating more or less liquid than what it is set to aspirate. What type of error is this?

You wish to study the effects of substrate concentration on enzyme rate. For the experiment, you alter the substrate concentration and measure the change in enzyme rate, while making sure the enzyme concentration is constant in each sample. Which is the dependent variable?

You measure a solution containing BSA (ε = 43,824 M-1 cm-1) in the spectrophotometer. The absorbance is 0.5. What is the concentration of BSA?

Which of the following is FALSE regarding the NIH grant review process?

100 ng of DNA is needed for a ligation, and its concentration is 45 ng/µl. How many microliters of DNA are required?

A protein has an absorbance of 0.36, and 4 tryptophans (ε = 5500) and 2 tyrosines (ε = 1490). What is its concentration?

An Office of Research administrator’s salary is paid out of the University’s budget. This is considered

Tris is a commonly used buffer between pH 7.5-9.0. Why is it not used above or below this range?

Which of the following statements about proteins is TRUE?

The serological pipette has a ________ tip because its purpose is ________ an exact amount for a single dispense.