You are a scientist in a biotechnology company that produces…

You are a scientist in a biotechnology company that produces custom engineered tissues for patients. Your current project is to develop a cartilage replacement for a 30-year-old athlete with osteoarthritic joints. At the team meeting, you propose using mesenchymal stem cells isolated from the patient for this project. A colleague suggests using stem cells isolated from the Wharton jelly of the umbilical cord. 1. What advantages (provide 2) does using the patient’s mesenchymal stem cells offer over the umbilical cord-derived cells? (4 points) 2. After discussions, your team decides to use the patient’s mesenchymal stem cells, what steps will you use to isolate these cells? Assume you have the bone marrow aspirates (6 points) 3. After initial test isolation, you perform a viability assay and discover that 80% of the isolated cells are dead. What could have caused this problem and how do you remedy it? (6 points; 3 points for the problem, 3 points for the solution)

Briefly describe the flow of water through a loam from a fur…

Briefly describe the flow of water through a loam from a furrow on the soil’s surface to an underlying clay layer. What happens to the water flow when it reaches the clay layer? Contrast this with what happens when the water encounters an underlying sand layer. If a pollutant were to be on the surface of the two soil profiles described above (loam with underlying clay versus loam with underlying sand layer), which would pose the greatest danger to groundwater? No more than 5 or 6 sentences!!