Author: Anonymous

Which technique was used to capture Taq-treated DNA for the sequencing of the human genome?

In the gel below, the DNA in Lane 3 is approximately

A sequencing gel has two clearly defined lanes for G and C, and then a purine and pyrimidine lane. This is likely

You wish to insert a gene into the vector below, grow the cells on ampicillin, and select colonies with blue/white screening. Which restriction enzymes should you use?  

When two sticky ends join, they make ________ between the bases, and ________ between the DNA backbones.

You design a PCR primer to a target sequence with an EcoRI overhang. The overhang bases (not the sequence-specific bases)

What is the Tm of the primer CGTAT?

You digest a ~2000 bp plasmid with EcoRI, and run a linear marker. The digestion at various time points is shown below. Which of the following does “B” represent?

Which molecule allows for real-time visualization of DNA during gel electrophoresis?

You digest a ~2000 bp plasmid with EcoRI, and run a linear marker. The digestion at various time points is shown below. Which of the following does “A” represent?