Author: Anonymous

When performing RT-PCR which of the following should be used as the reverse primer?

You design two primers with melting temperatures of 48 °C and 52 °C. If both of these primers were to be used together in PCR, what should the ideal annealing temperature be?

If you wanted to ensure correct directionality of a gene of interest being placed into a plasmid you would use 

If a laboratory yeast strain requires uracil to survive, it is considered a

Which of the following represents the correct order of a recombinant DNA experimental procedure?

RT-qPCR is similar to

Which of the following would you use to clone 300 kb of human DNA?

All of the following can typically hold 20,000 bp or more of DNA EXCEPT

You insert a gene into the vector below using the SalI and XbaI cut sites, grow the cells on ampicillin, and perform blue/white screening. Which is TRUE?

A piece of ssDNA from E. coli will hybridize with