Author: Anonymous

As you learned throughout MCB4034L, there are often pros and cons associated with each procedure.  Referring back to the previous question, even though the sensitivity is greater when using two antibodies, using just one antibody can be beneficial, assuming there is enough protein on the membrane.  Select the correct reason(s) from the choices below. There may be more than one correct answer.   

The plasmid isolation procedure we performed on three different occasions during the course has some similarities with the procedure we used to purify the recombinant GFP-CBM protein. Select from the options below the one correct answer.

The blocking buffer used as part of your western blot experiment contains non-fat milk powder purchased at a local supermarket. This is an inexpensive solution to obtaining which ingredient responsible for the blocking?

The desire to have satisfying relationships with others and feel a sense of love, affection, belonging, and acceptance is a reflection of _____ need.

_____ includes those marketing activities that provide extra value or incentives for purchasing a product, such as coupons and premiums.

You use your ligation reaction to transform E. coli Z-competent cells, plate them on LB medium containing IPTG (to provide access to the lac promoter), X-Gal (a colorless substrate for beta-galactosidase) and an antibiotic, and incubate them overnight at 37C. The next morning you see lots of colonies, both white and blue. The map of the pBluescript plasmid is available via the link below. pBluescript.pdf Select from the four choices below what the appropriate antibiotic in the LB medium is, and whether you should select white or blue colonies for the next step (colony PCR).

Your are getting ready to prepare two protein samples related to your snake venom project for PAGE. You plan to load 18 uL of sample A on the gel, and 24 uL of sample B. Before you can do this, you need to add the correct volume of loading dye, containing SDS, beta-mercaptoethanol and a blue pigment. The loading dye comes as a 4x concentrate. How much loading dye should you add to the 18 uL of sample A and to the 24 uL of sample B?

Which of the following is true of retail advertising?

Which of the following is considered as the final stage in the consumer decision-making process?

You’ve managed to get the snake gene expressed in a cell culture system and now need to harvest cells. This is performed by centrifugation. You were taught to always balance the rotor of the centrifuge, which includes matching the masses of the tubes exactly, and placing them in the proper position in the rotor. You have prepared two tubes with cells that have the same mass. When you are ready to spin these tubes, the first tube (light blue lid) goes in the spot shown below in the centrifuge rotor. Looking at the rotor from above, what is the correct rotor position for your second tube? Select the correct answer from the options below.