Author: Anonymous

Mass spectrometry identifies molecules based on what?

Based on what you know about CRISPR-Cas9, mention a real-life scenario where it would be beneficial to use this technology. Why would it be useful? (Be brief, but specific)

Which of these are not steps in a PCR reaction? (Select all that apply)

The activity of an enzyme is analyzed and it is found to have a Km value of 2.551 mM and a Vmax value of 0.174 mM/min. An inhibitor is introduced to the system, and the activity of the enzyme is re-analyzed. In the presence of this inhibitor, the enzyme is found to have a KM value of 2.551 mM and a Vmax value of 0.133 mM/min.  Based on the observed change in the enzyme’s activity, what kind of inhibitor do you believe is present in the system and why?

In your own words, briefly describe the CRISPR-Cas9 technology. 

During PCR in Next Gen Sequencing, one pair of primers is used to amplify all different sequences. 

Part of good lab practice is to identify the safety equipment around you and to label all samples with content, potential hazards, date and your initials.

Transcription occurs in the [answer1] while translation occurs in the [answer2] in organelles called [answer3].

A researcher is performing PCR to amplify a sample of DNA. Unfortunately, he forgot to add the DNA primer prior to starting the experiment. Briefly describe the results you expect he will observe and explain why you believe he will obtain these results. 

    The chromatogram pictured above is the result of a size exclusion chromatography experiment where five molecules eluted. List the molecules in order of size from largest to smallest and briefly explain how you are able to determine this.