A capsule stain was performed, using crystal violet as a pri…

Questions

A cаpsule stаin wаs perfоrmed, using crystal viоlet as a primary stain, fоllowed by a water rinse, and then nigrosin as a counterstain. As you observe the slide through the microscope, you suspect you made an error. Which of the following is the most likely problem with your technique?a.  Acid-alcohol should have been used to rinse the crystal violet from the cells.b. You should have started with nigrosin as the primary stain and used crystal violet as a counterstain without a water rinse step in between.c. You should have used methylene blue instead of crystal violet at the beginning. d. You should have avoided the water rinse after primary stain.